Center for Research in Multiscale Science and Engineering

Confocal laser scanning microscopy

Description

Confocal laser scanning microscopy is an optical imaging technique that allows sequential sample scan and that assembles the voxel information to one image.  The special pinhole blocks out-of-focus light giving this technique high resolution and contrast in X, Y and Z.  It is primarily used for examining cell and tissue cultures previously stained with fluorescent dyes. Our equipment is Carl ZEISS LSM 800.

Key features

  • Inverted stand: Axio Observer 7
  • Motorized XYZ stage
  • 4 diode lasers: 405, 488, 561 and 640 nm
  • 5 objectives: 5x, 10x and oil immersed 25x, 40x, 63x
  • Light and contrast manager: bright field, phase contrast, differential interface contrast, transmitted light techniques and epifluorescence techniques

Contact

In order to book the equipment, please contact Judit Buxadera ()